Immune System: at Teletest, We Have the most Advanced Technology in Flow Cytometry.

The Immune System is a complex network of cells known as immune cells. These cells have evolved and work together to protect our body from a wide variety of attacks from foreign substances / antigens, such as bacteria, fungi, parasites, viruses, etc., that are constantly in our environment and could alter our health.

Consequently, the immune response has developed two defense mechanisms: the innate response, which is the first line of defense against microbes, but is not specific and therefore not always effective, and the adaptive response, also called acquired, which is specific, and has immunological memory to respond more quickly to a subsequent antigenic exposure. Lymphocytes are responsible for mediating the adaptive immune response.

It is important that the levels of circulating lymphocytes in the blood are within an adequate range for the correct functioning of our immune system.

The evaluation of lymphocyte subpopulations or immunophenotype by flow cytometry is the most complete study to assess the status of the immune system.

Lymphocyte typing has become an indispensable tool in recent years for the diagnosis and monitoring of immunological, infectious, autoimmune, and oncological diseases. It consists of the detection, characterization, and quantification of the various lymphocyte populations according to their biological function through flow cytometry.

Flow cytometry is a high-precision technique for the classification and quantification of a cell suspension, based on the use of light beams. Cells are classified by their size and complexity, and thanks to specific markers called “clusters of differentiation” – CD, in the form of specific antibodies labeled with fluorochromes.

At Teletest, we have the most advanced technology in flow cytometry to perform a multiparametric analysis simultaneously, in absolute values for the detection of each specific cell type. We have an extensive catalog for the detailed study of the functioning of the immune system.

LYMPHOCYTE TYPING:

• ILMI or Lymphocyte Typing: Evaluate the state of inflammation / infection; Patients with recurrent Herpes infections; Patients with possible viral reactivations; Evaluate bacterial or parasitic infections; Profiles of patients with Fibromyalgia, Chronic Fatigue Syndrome.
• TH Status + Tregs: Determine levels of effector T CD4 Helper and T regulatory lymphocyte populations for diagnosis and monitoring of different immunological pathologies; Evaluate the inflammatory state, in response to bacterial or viral infections, tumors, intestinal diseases, skin diseases and in autoimmune processes; Study in response to allergies, asthma, helminths…
• NK Subpopulations+CD57: Type the NK subtypes and their level of activation; Lyme assessment
• Functional NK: Evaluate innate immunity by typing the functional activity of NK lymphocytes. Evaluate the balance between activating and inhibitory signals; Study of the state of activation / inhibition of Natural Killer cells; Applications in immunotherapy.
• Endometrial NK: Evaluate the infiltration of natural killer cells in endometrial tissue and determine the probability of infertility risk; Evaluate the balance between activating and inhibitory signals in the endometrium; Women with implantation failures, repetitive abortions, rule out an autoimmune origin in the endometrium.
• Checkpoints: Study the markers of the control points / pathways of the immune system; Evaluation of tumor, viral response control.
• B Status: Evaluation of B Lymphocyte subtypes; Study of the humoral response.
• Macrophages and Dendritic Cells (Macrophage Status): Evaluation of the regulation of macrophages, the main defense cells of innate immunity, to eliminate foreign bodies that penetrate our body (bacteria, parasites, viruses); Implication in Low Grade Inflammation.

IMMUNE STATUS (ILMI)

General evaluation of the state of our Immune System and its capacity to respond to different situations. Assessment of the state of inflammation / infection. We have the following analytical profiles:

• BASIC: Evaluation of the immune system and study of viral infections/reactivations or latent viruses.
• EXTENDED: Basic + inflammatory response.
• PLUS: Extended + study of effector T cells and cytokine production.
• Viral Status: Humoral response to latent viruses, assess infections and reactivations.
• Protein Status: Determines the profile of immunoglobulins, proteinogram and inflammatory proteins
• Th Status (+ Cytokin Status): Determines effector T cells and Tregs (state of inflammation) and quantifies pro-inflammatory and anti-inflammatory cytokines to evaluate the suitability of a treatment and the state of the immune system.

CELLULAR STATUS · CELLULAR RESPONSE

Evaluation of the immune response (cellular and humoral) by the production of INFy against the Antigens of the studied virus, to assess infection / reactivation or latent. We have the following analytical profiles:

• COVID: Humoral response; serology IgG (S1 and N), and IgM (S), Lymphocyte typing, Cellular response: T4, T8 and NK lymphocytes for M+N and S antigens separately.
• CMV: Serology, Avidity, Typing with differentiation of memory B cells and memory T CD8 and activation of Natural Killer cells, Cellular response: T4, T8 and NK lymphocytes.
• EBV: Serology, Typing with differentiation of memory B cells and memory T CD8 and activation of Natural Killer cells, Cellular response: T4, T8 and NK lymphocytes.
• Borrelia: Serology, Western-blot confirmation, Typing with differentiation of memory B cells and memory T CD8 and activation of Natural Killer cells, Cellular response: T4, T8 and NK lymphocytes.

CYTOKIN STATUS

Quantification of pro-inflammatory and anti-inflammatory cytokines to evaluate the suitability of a treatment and the state of the immune system.

Includes the determination of the following cytokines: Interferon gamma, GM-CSF. Granulocyte and macrophage stimulating factor, tumor necrosis factor, IL12p70, IL2, IL4, IL5, IL10, IL6, IL9, IL17A, interferon alpha.

In the ‘Immune System’ section of the website you can find all the information on each of the studies we carry out in Immunology. For any questions, you can contact us at: 932123345 – 912909973 or by email at laboratory@teletest.es.